Tuesday, December 24, 2019

Analysis Of The Book A Few Good Men By Solomon E. Asch

Comparative Analysis The foundation of today’s society is not in immense chaos or destruction, but rather in an organizational order because of the choice in obedience. As depicted in the movie, â€Å"A Few Good Men†, obedience is questioned due to soldiers choosing to obey or not when it presents the case of Lance Cpl. Harold Dawson and Pfc. Louden Downey being disciplined for committing a crime, even if they were only following orders. Eric Fromm, a social psychologist and psychoanalyst, furthers points in whether it is permissible to commit a crime under the pretext of obeying orders in his article, â€Å"Disobedience as a Psychological and Moral Problem†. Comparatively, Stanley Milgram, a Yale psychologist, addresses obedience overriding†¦show more content†¦Markinson disrupted social order by effectively disobeying Jessup, but Little and Fromm would coherently agree that the individualistic courage to disobey displayed by Markinson was extremely dif ficult and nearly impossible; however, this disobedience was greatly needed for an effective outcome. Although breaking social order is viewed as unsuitable and â€Å"out of character†, Brandt, an experimenter from Milgram’s research, demonstrated a clear break from authority which made disobedience a simple and rational deed (Milgram 80). Stephen Covey, an American educator, author, business man, and keynote speaker, wrote â€Å"The 7 Habits of Highly Effective People†, which puts rational deeds into fundamental concepts called principles (Covey 34). According to Covey, principles are self-evident natural laws and part of the human conscience that act as guidelines for human conduct which are proven to have enduring, permanent value; furthermore, obedience is declared a permanent principle (Covey 35). Markinson refutes this claim by telling the truth about Jessup’s intentions which displays someone’s ability to break a â€Å"permanent principleâ € . Milgram’s concept of disobedience acting as a principle contradicts with Covey’s more effective justification of obedience being a principle. In the end, Fromm and Milgram conclude that Markinson did have the choice to disobey, just as any other soldier would possess, even with the difficult struggles that LittleShow MoreRelatedStephen P. Robbins Timothy A. Judge (2011) Organizational Behaviour 15th Edition New Jersey: Prentice Hall393164 Words   |  1573 PagesOne Lake Street, Upper Saddle River, New Jersey 07458, or you may fax your request to 201-236-3290. Many of the designations by manufacturers and sellers to distinguish their products are claimed as trademarks. Where those designations appear in this book, and the publisher was aware of a trademark claim, the designations have been printed in initial caps or all caps. Library of Congress Cataloging-in-Publication Data Robbins, Stephen P. Organizational behavior / Stephen P. Robbins, Timothy A. JudgeRead MoreDeveloping Management Skills404131 Words   |  1617 PagesSuzanne Duda Lead Media Project Manager: Denise Vaughn Full-Service Project Management: Sharon Anderson/BookMasters, Inc. Composition: Integra Software Services Printer/Binder: Edwards Brothers Cover Printer: Coral Graphics Text Font: 10/12 Weidemann-Book Credits and acknowledgments borrowed from other sources and reproduced, with permission, in this textbook appear on appropriate page within text. Copyright  © 2011, 2007, 2005, 2002, 1998 Pearson Education, Inc., publishing as Prentice Hall, One

Monday, December 16, 2019

Hasbro in Brazil Free Essays

With 41. 9% of Hasbro sales revenue being generated internationally, it is essential that they work to maintain positive relationships with countries throughout the world. The international arena is becoming increasingly important to their revenues. We will write a custom essay sample on Hasbro in Brazil or any similar topic only for you Order Now Brazil is one of several international markets that Hasbro has expanded into over the last 5 – 10 years. Not only are they shipping products to Brazil, but in around 2007 they also opened an office in Brazil. Even with this being the case, Brazil has taken governmental measures to promote and strengthen the national traditional toys and games industry and stimulate further local production. In 2010, Brazil approved the reduction of import taxes from 20% to 2% on components to produce traditional toys and games such as electronic mechanisms and later the government increased import taxes on manufactured products from 20% to 35% to inhibit further imports, mainly from China (Toys and Games in Brazil, 2011). In a bid to remain competitive and increase market share, seven large domestic companies considered the possibility of merging to form the largest toys and game manufacturer in Brazil. While the project is still under consideration despite the challenges involved merging seven companies, three other companies, Baby Brink, Rosita and Acalanto, merged in April 2010 to create the group BBRA† (Toys and Games in Brazil, 2011). With measures such as these in place, it causes a disfavor t o companies such as Hasbro. It requires them has to work twice as hard to gain acceptance in a country that is promoting growth for their national companies. Hasbro identified one opportunity for the locals of Brazil to view them in a light other than one of an â€Å"outsider. † In 2011, Hasbro partnered with Operation Smile Create Smiles to provide free medical treatment and toys and games for children born with cleft palate. â€Å"The medical mission in Brazil was made possible because of Hasbro. Our patients and families in Brazil are incredibly warm and vibrant, and yet the laughter and smiles of these children were hidden behind their clefts. Today, their futures are full of hope and opportunity, and they will always remember that Hasbro helped make that happen†, said Dr. Bill Magee, Co-Founder and Executive Chairman of Operation Smile (Kraft, Jessica, 2011). By taking steps to increase their social responsibility in Brazil, Hasbro is promoting themselves in a way that will allow them to gain an increased acceptance rating and hopefully, not only make 100 children’s smiles brighter but also their bottom line. How to cite Hasbro in Brazil, Essay examples

Sunday, December 8, 2019

Isolation And Characterization Of DNA From Onion Biology Essay Example For Students

Isolation And Characterization Of DNA From Onion Biology Essay The experiment was about the isolation and word picture of DNA. The Deoxyribonucleic acid was isolated from the onion. The mass of the stray DNA was 15.11 g. The pureness of stray DNA was estimated by ciphering the ratio based from the optical density at 260nm and 280nm resulted to 0.671 intending more protein was absorbed. Meanwhile in denaturation of DNA, the initial optical density at 260 nanometer was 1.304 higher than the optical density at 260 nanometers after heating which was 1.095. Outline1 Introduction2 MATERIALS AND METHODS3 Isolation of Deoxyribonucleic acid from Onion4 Word picture of DNA.5 RESULTS AND DISCUSSIONS6 Calculation 1. Solution for Percentage Yield Determination7 Figure 1. Optical density at 700 nanometers Introduction Deoxyribonucleic acid ( DNA ) is the familial stuff in worlds and all other beings. DNA isolation is the remotion of Deoxyribonucleic acid from the cell which it usually resides. Isolation is the remotion of Deoxyribonucleic acid from the cell in which it usually inhabits. ( 1 ) Onions are used since it contains small sum of amylum which allows the Deoxyribonucleic acid to be more seeable. The filtrate is made up of onions treated with salt, distilled H2O and detergent jointly called as lysis solution. DNA purification is done by enzymatic debasement of polluting proteins with ethyl alcohol. A spectrophotometer is used in finding the concentration and pureness of the proteins. ( 2 ) MATERIALS AND METHODS Isolation of Deoxyribonucleic acid from Onion The bare-assed onion bulb was chopped and measured homogenized. The sample was placed in a liquidizer added with an ice-cold lysis solution so for 45 seconds at low velocity. Meanwhile, the lysis solution used was prepared beforehand by blending 5.00 milliliter of liquid detergent, 5.00 milliliter of 0.500M EDTA, 10.0 milliliter of 50 % Na Cl solution, and 80 milliliter of distilled H2O and placed in an ice bath. After homogenising, the sample was filtered through the cheesecloth and the gathered filtrate was placed in a 250-ml beaker. A 10.0 milliliter of 5 % pepsin solution was added to the filtrate and placed on an ice bath for 10 proceedingss with occasional stirring. Ice cold 30.0 milliliter of 95 % ethyl alcohol was pipette to the side of the beaker incorporating the sample and base for 10 proceedingss on ice bath. Once the Deoxyribonucleic acid precipitates appeared at the interface of the solution, the Deoxyribonucleic acid was already ready for isolation. The spooled Deoxyri bonucleic acid was transferred instantly to a pre-weighed 100-ml beaker to find the mass and percent output of the sample. The stray DNA was added with 10.0 milliliters of 95 % ethyl alcohol so covered with aluminium foil and refrigerated in readying for the following research lab process. Word picture of DNA. Small sum of DNA sample was placed in a trial tubing added with 1.00 milliliters of 20 % TCA followed by heating the sample for 10 proceedingss in H2O bath with 1.00 milliliters distilled H2O. A 2.00 milliliter of diphenylamine solution was added so heat once more in a H2O bath for 10 proceedingss. The colour alteration was observed and the optical density of the sample from 400 nanometers to 700 nanometer was scanned to find the wavelength of maximal soaking up. Mean while, small sum of the DNA sample was placed in a separate trial tubing filled with 5.00 milliliters distilled H2O and scanned to read the optical density at 260 nm so at 280 nanometer. After finding the A260/A280 value, the sample was heated to boil for 5 proceedingss and read the optical density adain at 260 nanometers. RESULTS AND DISCUSSIONS The mass of the natural sample gathered from onion is 30.4 g. After homogenisation and adding of pepsin solution and ethyl alcohol, DNA precipitates were became seeable and transferred to another beaker. The stray DNA measures 23 g. .ucb6bd25696e569409fa7fd76553b308b , .ucb6bd25696e569409fa7fd76553b308b .postImageUrl , .ucb6bd25696e569409fa7fd76553b308b .centered-text-area { min-height: 80px; position: relative; } .ucb6bd25696e569409fa7fd76553b308b , .ucb6bd25696e569409fa7fd76553b308b:hover , .ucb6bd25696e569409fa7fd76553b308b:visited , .ucb6bd25696e569409fa7fd76553b308b:active { border:0!important; } .ucb6bd25696e569409fa7fd76553b308b .clearfix:after { content: ""; display: table; clear: both; } .ucb6bd25696e569409fa7fd76553b308b { display: block; transition: background-color 250ms; webkit-transition: background-color 250ms; width: 100%; opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #95A5A6; } .ucb6bd25696e569409fa7fd76553b308b:active , .ucb6bd25696e569409fa7fd76553b308b:hover { opacity: 1; transition: opacity 250ms; webkit-transition: opacity 250ms; background-color: #2C3E50; } .ucb6bd25696e569409fa7fd76553b308b .centered-text-area { width: 100%; position: relative ; } .ucb6bd25696e569409fa7fd76553b308b .ctaText { border-bottom: 0 solid #fff; color: #2980B9; font-size: 16px; font-weight: bold; margin: 0; padding: 0; text-decoration: underline; } .ucb6bd25696e569409fa7fd76553b308b .postTitle { color: #FFFFFF; font-size: 16px; font-weight: 600; margin: 0; padding: 0; width: 100%; } .ucb6bd25696e569409fa7fd76553b308b .ctaButton { background-color: #7F8C8D!important; color: #2980B9; border: none; border-radius: 3px; box-shadow: none; font-size: 14px; font-weight: bold; line-height: 26px; moz-border-radius: 3px; text-align: center; text-decoration: none; text-shadow: none; width: 80px; min-height: 80px; background: url(https://artscolumbia.org/wp-content/plugins/intelly-related-posts/assets/images/simple-arrow.png)no-repeat; position: absolute; right: 0; top: 0; } .ucb6bd25696e569409fa7fd76553b308b:hover .ctaButton { background-color: #34495E!important; } .ucb6bd25696e569409fa7fd76553b308b .centered-text { display: table; height: 80px; padding-left : 18px; top: 0; } .ucb6bd25696e569409fa7fd76553b308b .ucb6bd25696e569409fa7fd76553b308b-content { display: table-cell; margin: 0; padding: 0; padding-right: 108px; position: relative; vertical-align: middle; width: 100%; } .ucb6bd25696e569409fa7fd76553b308b:after { content: ""; display: block; clear: both; } READ: Thomas Edison , A Great Inventor In 20Th Century EssayPercentage Recovery Mass or natural sample: 30.4 g Mass of stray Deoxyribonucleic acid: 15. 11 g Percent Output: 49.70 % Word picture of Deoxyribonucleic acid Chemical reaction with Diphenylamine Color of formed solution: green Experimental A ; Ucirc ; ?max: Theoretical A ; Ucirc ; ? soap: Percentage mistake: Purity Determination Optical density at 250 nanometers: 1.304 Optical density at 280 nanometers: 1.942 A260/ A280 value: 0.671 Denaturation of Deoxyribonucleic acid Initial optical density at 260 nanometers: 1.304 Optical density at 260 nanometers after warming: 1.095 Percent addition in optical density: 8 % Calculation 1. Solution for Percentage Yield Determination The deliberate per centum output was rather high. However, still some beginnings of mistake was done while carry oning the experiment, the sample with DNA precipitates was disturbed while reassigning the Deoxyribonucleic acid. The accrued DNA precipitates is adequate for the following process which is word picture. Figure 1. Optical density at 700 nanometers Heat denaturation of DNA, causes the dual spiral construction to wind off and organize individual stranded Deoxyribonucleic acid. Therefore, the bases unstacked and can absorb more light doing an addition after denaturation. But based on the consequences gathered, the initial optical density at 260 nanometer was 1.304 so was decreased after heating which was 1.095. The deliberate per centum addition in optical density was 8 % . This mistake is possibly, due to the warming procedure. The Deoxyribonucleic acid acquired was rather greater and was non wholly het afterwards doing dual spiral construction non to wind off and organize a individual isolated Deoxyribonucleic acid. The filtrate gathered from this experiment was made of onions and lysis solution. Onion was used in this survey due to low amylum content, leting the Deoxyribonucleic acid to be more seeable sing the onion as one of the best beginning of DNA. ( 4 ) The used of lysis solution was to divide the Deoxyribonucleic acid from excess cell constituents and to maintain the location in which the Deoxyribonucleic acid will non be tainted. The NaCL provides NA+ ions that will blockade the negative charge as of phosphate terminals of DNA. Permiting these terminals to come nigher so they can precipitate out of a cold solution. The detergent causes the interrupting down of the cell membrane by emulsifying the cell proteins and lipoids. Besides, interrupting the polar connexions that jointly holds the cell membrane. The composites formed with these lipoids and proteins causes the precipitate out of solution. Meanwhile, the intent of EDTA is to chelates metal ions. ( 5 ) A Pepsin solution was used for purification via enzymatic debasement. Deoxyribonucleic acid is polar due to its highly charged phosphate anchor which makes it soluble in H2O. Thus DNA is indissoluble in ice cold ethyl alcohol, as a consequence when the cold ethyl alcohol was added, it causes stable ionic bonds to organize and precipitate the Deoxyribonucleic acid. Heating the sample is the 1 responsible for the formation of the ascertained colour of Deoxyribonucleic acid with diphenylamine. When the Deoxyribonucleic acid is heated with acid, the 2-deoxyribose is converted to w-hydroxylaevulinic aldehyde, which reacts with the compound diphenylamine. Through this, a blue-colored compound supposed to bring forth. In our sample the colour observed was green perchance because of the DNA concentration. The ratio of soaking ups at 260 nm V 280 nanometer is often used to measure DNA taint of protein solutions. The nucleic acids, DNA and RNA, absorbs at 260 nanometers and proteins absorb at 280 nanometer. Based on the consequences, the rate ratio of soaking ups at 260 nm V 280 nanometer is 0.671. Since proteins absorb visible radiation at 280 nanometer, the ratio is low intending there is a batch of protein absorbed at 280nm.